ABSTRACT
Fingerprints of 18 batches of substance benchmark of Shentong Zhuyu Decoction(SZD) were established by UPLC under the following conditions: Waters Sun Fire C_(18) column(3.0 mm×150 mm, 3.5 μm), column temperature of 35 ℃, gradient elution with mobile phase of acetonitrile(A)-0.1% phosphoric acid aqueous solution(B) at the flow rate of 0.4 mL·min~(-1), and detection by wavelength switching. A total of 16 common peaks were identified. The similarities among the fingerprints were calculated by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition) and the result showed they were in the range of 0.911-0.988. Based on the 16 common peaks, cluster analysis(CA), principal component analysis(PCA), and partial least square discriminant analysis(PLS-DA) all categorized the 18 batches of samples into two groups(S1, S2, S5-S8, S14, and S17 in one group, and S1, S2, S5-S8, S14, and S17 in another), and 11 most influential components were screened. Five known components with great difference among samples(hydroxysafflor yellow A, ferulic acid, benzoic acid, ecdysone, and ammonium glycyrrhizinate) were determined. The combination of multi-component content determination and fingerprints can reflect the overall cha-racteristics of the primary standards of SZD, which is simple, feasible, reproducible, and stable. This study can serve as a reference for the quality control of the primary standards of SZD.
Subject(s)
Chromatography, High Pressure Liquid , Drugs, Chinese Herbal/standards , Quality ControlABSTRACT
Objective: To optimize the condition of the fingerprint of the classic prescription Shentong Zhuyu Decoction (SZD) by the principle of information entropy maximization. Methods: The chromatographic fingerprint was obtained with Acquity UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm) and gradient eluted with acetonitrile and 0.4% phosphoric acid aqueous solution; The flow rate was 0.6 mL/min, and the column temperature was maintained at 40 ℃; The detection wavelengths were set at 190-400 nm, and the injection volume was 1 μL, and calculate the information entropy to optimize the wavelength of maximizing the information entropy of the fingerprint of SZD, under the optimal wavelength, investigate the mobile phase, column temperature, flow rate, sample volume, select the optimal conditions based on the principle of maximizing the information entropy, and carry out the fingerprint study of 15 batches of SZD under the optimal conditions. Results : Under the principle of information entropy maximization, the optimal conditions of the fingerprint of SZD were as follows: the detection wavelength was 235 nm, and gradient eluted with acetonitrile and 0.4% phosphoric acid aqueous solution; The flow rate was 0.4 mL/min, and the column temperature was maintained at 40 ℃, the injection volume was 3 μL; The absolute and relative entropy was the largest. The total statistical moment similarity of 15 batches of SZD fingerprints ranged from 0.882 8 to 0.995 3. The RSD values of the total first-order moment and information entropy of the total amount were 5.40% and 2.25% respectively, all of which were less than 10%, indicating that the imprinting effect of the overall chemical structure of the multi-component system of traditional Chinese medicine was stable. Conclusion: When the number and area of chromatographic peaks reach the best characterization, the information entropy is the largest. At this time, the number of peaks is high, the shape of peaks is better, the resolution is good, the number of theoretical plates is higher, and the best condition of fingerprint can be determined. The principle of information entropy maximization can provide scientific basis for fingerprint optimization of traditional Chinese medicine and its compound prescription, and lay a foundation for quality control of classical formulas.
ABSTRACT
Objective To investigate the efficacy of a Chinese Medicine formula on chemotherapy-induced peripheral neuropathy (CIPN).Methods From April,2013 to December,2014,56 patients were randomized blindly to receive granulas of Chinese Medicine of modified Huangqi-Guizhi Wuwu and Shentong-Zhuyu(treatment group,n=28)or placebo(control group,n=28),for a month.They were as-sessed with Number Rating Scale (NRS) of symptoms of CIPN, National Cancer Institute's Common Terminology Criteria for Adverse Events (NCI-CTCAE) version 4.0, and European Organization for Research and Treatment of Cancer Quality of Life Questionnaire-30 (EORTC QLQ-C30)before,and four weeks,eight weeks and twelve weeks after treatment.Results The score of NRS(F>9.518,P<0.01) and grade of NCI-CTCAE(Z>3.887,P<0.05)improved in the treatment group compared with those in the control group eight weeks and twelve weeks after treatment.The scores of EORTC QLQ-C30 also improved twelve weeks after treatment, but only significantly in the symptom of fatigue(t=-2.971,P<0.01).Conclusion The Chinese Medicine can release the symptoms of CIPN,decrease the grade of neuro-toxicity,and may improve the quality of life somewhat.
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Objective To study the effects of ultrafiltration process on activating blood and removing blood stasis efficacy ofShentong Zhuyu Decoction; To investigate the feasibility of applying ultrafiltration technology in purifying Shentong Zhuyu Decoction.Methods The mice micro artery and vein diameter, clotting time and opening capillary of auricle microcirculation of mice were used as indexes to observe the effects of different ultrafiltration process on activating blood and removing blood stasis efficacy ofShentong Zhuyu Decoction.ResultsShentong Zhuyu Decoction showed satisfying efficacy of activating blood and removing blood stasis. There was no significant difference between the non-ultrafiltration process and ultrafiltration processed by 20 and 50 nm ultrafiltration membranes.Conclusion Ultrafiltration technology can be applied to purifying Shentong Zhuyu Decoction, and the membrane pore size must be more than 20 nm.